Enzymes
|
Metabolic
steps
|
Activity
normal conditions
|
Activity
paraquat treated
|
PQ/normal Ratios
|
---|
IDH
|
icd
|
0.301 ± 0.009
|
0.155 ± 0.010
|
0.51
|
AKGDH
|
akd
|
0.191 ± 0.008
|
0.090 ± 0.015
|
0.47
|
MDH
|
mdh
|
0.106 ± 0.003
|
0.303 ± 0.013
|
2.86
|
G6PDH
|
gdh
|
0.428 ± 0.000
|
1.219 ± 0.011
|
2.85
|
PGI
|
hxi
|
0.207 ± 0.003
|
0.297 ± 0.007
|
1.44
|
ICL
|
gs1
|
0.787 ± 0.012
|
1.506 ± 0.004
|
1.91
|
ENO
|
eno
|
0.072 ± 0.012
|
0.072 ± 0.013
|
1.00
|
THD
|
-
|
0.122 ± 0.005
|
0.181 ± 0.003
|
1.48
|
- aEnzyme activities in cell extracts of control or paraquat-treated wildtype E. coli JM101 grown on glucose at D = 0.17 h-1 under aerobic chemostat conditions. Ratio calculation was based on control conditions (wildtype aerobic growth on glucose). The unit of enzyme activity is μmol min-1 (mg protein) -1. All measurements were performed in triplicate. Data are expressed as means ± S.D. IDH: isocitrate dehydrogenase NADP-dependent, AKGDH: α-ketoglutaric acid dehydrogenase, PGI: glucose phosphate isomerase, MDH: malate dehydrogenase, G6PDH: glucose-6-phosphate dehydrogenase, ICL: isocitrate lyase, ENO: enolase, THD: transhydrogenase.