Enzymes | Metabolic steps | Activity normal conditions | Activity paraquat treated | PQ/normal Ratios |
|---|
IDH | icd | 0.301 ± 0.009 | 0.155 ± 0.010 | 0.51 |
AKGDH | akd | 0.191 ± 0.008 | 0.090 ± 0.015 | 0.47 |
MDH | mdh | 0.106 ± 0.003 | 0.303 ± 0.013 | 2.86 |
G6PDH | gdh | 0.428 ± 0.000 | 1.219 ± 0.011 | 2.85 |
PGI | hxi | 0.207 ± 0.003 | 0.297 ± 0.007 | 1.44 |
ICL | gs1 | 0.787 ± 0.012 | 1.506 ± 0.004 | 1.91 |
ENO | eno | 0.072 ± 0.012 | 0.072 ± 0.013 | 1.00 |
THD | - | 0.122 ± 0.005 | 0.181 ± 0.003 | 1.48 |
- aEnzyme activities in cell extracts of control or paraquat-treated wildtype E. coli JM101 grown on glucose at D = 0.17 h-1 under aerobic chemostat conditions. Ratio calculation was based on control conditions (wildtype aerobic growth on glucose). The unit of enzyme activity is μmol min-1 (mg protein) -1. All measurements were performed in triplicate. Data are expressed as means ± S.D. IDH: isocitrate dehydrogenase NADP-dependent, AKGDH: α-ketoglutaric acid dehydrogenase, PGI: glucose phosphate isomerase, MDH: malate dehydrogenase, G6PDH: glucose-6-phosphate dehydrogenase, ICL: isocitrate lyase, ENO: enolase, THD: transhydrogenase.
