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Table 5 Validation of predicted meiotic genes by sporulation assay

From: A yeast's eye view of mammalian reproduction: cross-species gene co-expression in meiotic prophase

 

Strain

Gene

% cells completing meiosis I*

% cells completing meiosis II#

Positive control

BY4743

-

18.3%

17.7%

Negative control

BY4743 rim11

RIM11

0%

0%

 

BY4743 rim15

RIM15

0%

0%

Predicted meiotic genes

BY4743 doc1

DOC1

3.2%

0.4%

 

BY4743 ste24

STE24

5.3%

4.7%

 

BY4743 ydr374c

YDR374C

4.5%

4.5%

 

BY4743 cog6

COG6

13.6%

13.0%

 

BY4743 gos1

GOS1

8.2%

7.2%

 

BY4743 nif3

NIF3

12.4%

10.9%

  1. * Percentage of binucleates, trinucleates, and tetranucleates among 300 cells for each strain.
  2. # Percentage of trinucleates and tetranucleates among 300 cells for each strain.