From: A multi-level study of recombinant Pichia pastoris in different oxygen conditions
% Fraction of total pool | Expressing Strain | Control Strain | ||||
---|---|---|---|---|---|---|
 | 21% O2 | 11% O2 | 8% O2 | 21% O2 | 11% O2 | 8% O2 |
Pep from PPs, upper bound | 50 ± 9 | 23 ± 6 | 15 ± 7 | 39 ± 9 | 32 ± 8 | 15 ± 6 |
R5P from T3P and S7P (transketolase) | 71 ± 2 | 78 ± 2 | 70 ± 2 | 66 ± 2 | 70 ± 2 | 62 ± 2 |
R5P from E4P (transaldolase) | 44 ± 2 | 24 ± 2 | 23 ± 2 | 40 ± 2 | 29 ± 2 | 24 ± 2 |
Ser originating from Gly and C1-unit | 61 ± 4 | 68 ± 4 | 68 ± 4 | 62 ± 4 | 69 ± 4 | 72 ± 4 |
Gly originating from CO2 and C1-unit | 10 ± 4 | 13 ± 3 | 13 ± 3 | 6 ± 4 | 12 ± 3 | 10 ± 3 |
Pep originating from Oaacyt (PepCK) | 0 ± 4 | 0 ± 8 | 0 ± 10 | 2 ± 5 | 0 ± 10 | 5 ± 11 |
Oaamit originating from Pep | 44 ± 2 | 32 ± 2 | 44 ± 2 | 42 ± 2 | 35 ± 2 | 41 ± 2 |
Oaa mit originating from Oaa cyt | n.a. | 43 ± 3 | 55 ± 3 | n.a. | 44 ± 3 | 51 ± 3 |
Oaa cyt originating from Pep | n.a. | 63 ± 3 | 64 ± 4 | n.a. | 66 ± 3 | 66 ± 4 |
Oaacyt reversibly converted to Fum | 63 ± 11 | 7 ± 5 | 10 ± 5 | 63 ± 11 | 11 ± 4 | 9 ± 4 |
Flux through malic enzyme, upper bound | 1 ± 4 | n.d. | n.d. | 1 ± 6 | n.d. | n.d. |
Flux through malic enzyme, lower bound | 1 ± 2 | n.d. | n.d. | 0 ± 3 | n.d. | n.d. |