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Table 5 Metabolic flux ratio (METAFoR) analysis results

From: A multi-level study of recombinant Pichia pastoris in different oxygen conditions

% Fraction of total pool

Expressing Strain

Control Strain

 

21% O2

11% O2

8% O2

21% O2

11% O2

8% O2

Pep from PPs, upper bound

50 ± 9

23 ± 6

15 ± 7

39 ± 9

32 ± 8

15 ± 6

R5P from T3P and S7P (transketolase)

71 ± 2

78 ± 2

70 ± 2

66 ± 2

70 ± 2

62 ± 2

R5P from E4P (transaldolase)

44 ± 2

24 ± 2

23 ± 2

40 ± 2

29 ± 2

24 ± 2

Ser originating from Gly and C1-unit

61 ± 4

68 ± 4

68 ± 4

62 ± 4

69 ± 4

72 ± 4

Gly originating from CO2 and C1-unit

10 ± 4

13 ± 3

13 ± 3

6 ± 4

12 ± 3

10 ± 3

Pep originating from Oaacyt (PepCK)

0 ± 4

0 ± 8

0 ± 10

2 ± 5

0 ± 10

5 ± 11

Oaamit originating from Pep

44 ± 2

32 ± 2

44 ± 2

42 ± 2

35 ± 2

41 ± 2

Oaa mit originating from Oaa cyt

n.a.

43 ± 3

55 ± 3

n.a.

44 ± 3

51 ± 3

Oaa cyt originating from Pep

n.a.

63 ± 3

64 ± 4

n.a.

66 ± 3

66 ± 4

Oaacyt reversibly converted to Fum

63 ± 11

7 ± 5

10 ± 5

63 ± 11

11 ± 4

9 ± 4

Flux through malic enzyme, upper bound

1 ± 4

n.d.

n.d.

1 ± 6

n.d.

n.d.

Flux through malic enzyme, lower bound

1 ± 2

n.d.

n.d.

0 ± 3

n.d.

n.d.

  1. Origins of metabolic intermediates during growth of P. pastoris in glucose-limited chemostat 13C-labelled cultures at D = 0.1 h-1 at different fractions of oxygen in the chemostat inlet gas. Values for both control and Fab producing strains are given. Ratios highlighted in bold have been used as constraints for metabolic flux analysis. n.a., not applicable; n.d., not determined. See main text for explanation.