Figure 2

Steady state distribution of iron content in ferritin. (A) In vitro steady state distribution of iron content in ferritin. Horse spleen apoferritin was loaded with different iron to ferritin ratios of 300:1, 450:1, 1000:1, 1300:1 and 1800:1 [44] and loaded into a sucrose gradient (1-25%). Fractions were collected and ferritin content was determined by the BCA assay. The iron content was calculated using the calibration curve in Figure 1; (B) Simulation results for the steady state distribution of iron content in ferritin for iron to ferritin ratios of 300:1, 450:1, 1000:1, 1300:1 and 1800:1. The iron content distribution was transformed to sedimentation fractions using the calibration curve in Figure 1, allowing for the recovery of the distributions observed experimentally. Iron packages were transformed to iron atoms (50 atoms per package) and then associated to a corresponding experimental fraction.