Pattern of EV along the signaling hierarchy. A) A visual depiction of the signaling hierarchy as defined in text and Methods. B) Network plots of the signaling hierarchy. Each layer corresponds to the corresponding layer in A. Node colors show EVexpo values of corresponding genes and lines indicate directed protein-protein interactions. C) Heatmap of enrichment p-values of each gene class as defined in Kim et al (2005) for genes in corresponding signaling hierarchy levels. Colors indicate negative log (base 10) of p-values as determined by hypergeometric distribution formula. D) Boxplots of EV values of RS level genes with class I, II and III promoters. P-values of difference were calculated by Wilcoxon rank sum test.