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Table 1 A-stat and chemostat growth characteristics comparison and A-stat reproducibility over the studied specific growth rate range for three independent experiments.

From: Systems biology approach reveals that overflow metabolism of acetate in Escherichia coli is triggered by carbon catabolite repression of acetyl-CoA synthetase

  μ = 0.24 h-1 μ = 0.30 h-1 μ = 0.40 h-1 μ = 0.51 h-1 μ = 0.10-0.47 h-1
  Chemostat A-stat Chemostat A-stat Chemostat A-stat Chemostat A-stat A-stat RSD, %
YXSa 0.44 0.40 ± 0.01 0.46 0.41 ± 0.01 0.44 0.42 ± 0.00 0.43 0.41 ± 0.01 2.0
YOAc-b NDE NDE 0.53 0.90 ± 0.32 1.70 1.56 ± 0.23 3.25 3.35 ± 0.82 ND
YcAMPc 3.47 3.59 ± 0.39 3.25 3.55 ± 0.32 2.70 2.17 ± 0.07 0.86 0.71e 9.1
YCO2d 27.56 30.12 ± 2.04 27.55 27.19 ± 1.22 26.24 23.86 ± 1.41 ND 21.19 ± 0.19 5.6
  1. A-stat values represent the average from three independent experiments and standard deviation follows the ± sign. Chemostat values from one experiment. NDE, not detected. ND, not determined. RSD, relative standard deviation.
  2. aBiomass yield is given in g dry cell weight (DCW)/g glucose consumed (g DCW/g glucose).
  3. bAcetic acid production per biomass is given in mmol acetic acid/g DCW.
  4. ccAMP production per biomass is given in μmol cAMP/g DCW.
  5. dCarbon dioxide (CO2) production per biomass is given in mmol CO2/g DCW.
  6. eData from one A-stat experiment.