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Table 1 A-stat and chemostat growth characteristics comparison and A-stat reproducibility over the studied specific growth rate range for three independent experiments.

From: Systems biology approach reveals that overflow metabolism of acetate in Escherichia coli is triggered by carbon catabolite repression of acetyl-CoA synthetase

 

μ = 0.24 h-1

μ = 0.30 h-1

μ = 0.40 h-1

μ = 0.51 h-1

μ = 0.10-0.47 h-1

 

Chemostat

A-stat

Chemostat

A-stat

Chemostat

A-stat

Chemostat

A-stat

A-stat RSD, %

YXSa

0.44

0.40 ± 0.01

0.46

0.41 ± 0.01

0.44

0.42 ± 0.00

0.43

0.41 ± 0.01

2.0

YOAc-b

NDE

NDE

0.53

0.90 ± 0.32

1.70

1.56 ± 0.23

3.25

3.35 ± 0.82

ND

YcAMPc

3.47

3.59 ± 0.39

3.25

3.55 ± 0.32

2.70

2.17 ± 0.07

0.86

0.71e

9.1

YCO2d

27.56

30.12 ± 2.04

27.55

27.19 ± 1.22

26.24

23.86 ± 1.41

ND

21.19 ± 0.19

5.6

  1. A-stat values represent the average from three independent experiments and standard deviation follows the ± sign. Chemostat values from one experiment. NDE, not detected. ND, not determined. RSD, relative standard deviation.
  2. aBiomass yield is given in g dry cell weight (DCW)/g glucose consumed (g DCW/g glucose).
  3. bAcetic acid production per biomass is given in mmol acetic acid/g DCW.
  4. ccAMP production per biomass is given in μmol cAMP/g DCW.
  5. dCarbon dioxide (CO2) production per biomass is given in mmol CO2/g DCW.
  6. eData from one A-stat experiment.