Figure 1

Anti-IgM-dependent stimulation of CH1 cells leads to a poor activation of the signaling responses and induces cell cycle arrest. Panel A shows the histograms representing the distribution of cells in each phase of the cell cycle observed by FACS analysis performed 16 hours after a 1 h stimulation of CH1 cells with anti-IgM. An arrest in cell cycle is clearly evident here, with a greater than 2-fold increase in the G1 phase population. Also shown in the inset is the result of a Western blot analysis revealing accumulation of p27 upon anti-IgM stimulation over the course of time. Panel B depicts the activation profiles of fourteen signaling intermediates probed by Western blots (see text for details and Additional File 1: Supplemental Fig. S1A) after anti-IgM stimulation. The plot represents mean value of quantified, normalized fold change in phosphorylation of the signaling intermediates in a time dependant manner obtained from three individual replicates and the over all S.D observed was < 10%. Also refer Additional File 1: Supplemental Fig. S1B for a detailed plot of the figure. An Ingenuity Pathway Analysis (IPA) of the set of early induced genes upon anti-IgM stimulation of CH1 cells is shown in Panel C. This network depicts the core module identified by IPA based on the list of early-induced genes used as the seed nodes. Here, the nodes in red represent the over-expressed genes while those in green are those whose expression was found to suppressed (Detailed key in Additional file 1). The canonical pathways identified by IPA for the set of early induced genes in CH1 cells and their corresponding significant levels of contribution by the early induced genes is shown in Panel D.