Figure 5
Delineating the core molecular constituents of the BCR-dependent pathways mediating cell cycle arrest. Panel A depicts the time dependent phosphorylation status of signaling intermediates upon anti-IgM stimulation in presence of p38 inhibitor SB203580 and CaMKII inhibitor KN62. Values are mean of three individual experiments with ± S.D. Modulation of Transcription factor activities upon BCR stimulation in the presence of SB203580 and KN62 is shown in panel B. The TF array blots after quantification and normalization are represented as heat map to represents the fold changes in TF activity expressed on a log10 scale. Panel C shows the activity profiles of seven TFs short listed based on their effect of early-induced genes in presence of the p38 and CaMKII inhibitors (see text for details). Shown here are the actual quantitative numbers obtained after image quantification.