Skip to main content
Figure 12 | BMC Systems Biology

Figure 12

From: Two additive mechanisms impair the differentiation of 'substrate-selective' p38 inhibitors from classical p38 inhibitors in vitro

Figure 12

Protein expression levels. A, sample Western blots used for quantitation of total p38α and MK2 protein levels. On these representative blots, U937 cell lysates from 3 different experiments (lanes [14] and [5, 6]) are loaded at 5 ug total protein (lanes 1, 3, 5) and 2 ug total protein (lanes 2, 4, 6), as measured by BCA assay. Recombinant p38-alpha and MK2 standards were loaded using 3-fold dilutions starting from 1.68 × 1010 and 1.34 × 1011 molecules per lanes, respectively. Cell lysates from U937 (n = 11, 13, for p38 and MK2 blots, respectively), Thp-1 (n = 4, 3) and PBMCs (n = 3, 4) were measured. Images were quantified with densitometry and total p38α (open bars) and MK2 (filled bars) protein expression levels were calculated. Results are shown in B, # of molecules/cell and in C, uM. Data is shown ± standard deviation.

Back to article page