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Figure 2 | BMC Systems Biology

Figure 2

From: Two additive mechanisms impair the differentiation of 'substrate-selective' p38 inhibitors from classical p38 inhibitors in vitro

Figure 2

Dual substrate assay. A, the time course of MK2 phosphorylation by p38 is measured in the presence (□) or absence (Δ) of 100 nM ATF2. No MK2 (ATF2 only, ) and no enzyme (×) are shown as controls. B, the time course of ATF2 phosphorylation by p38 is measured in the presence (□) or absence () of 10 nM MK2. No ATF2 (MK2 only, Δ) and no enzyme (×) are shown as controls. Data is shown ± standard deviation (n = 4).

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