Figure 2

Dual substrate assay. A, the time course of MK2 phosphorylation by p38 is measured in the presence (□) or absence (Δ) of 100 nM ATF2. No MK2 (ATF2 only, ▽) and no enzyme (×) are shown as controls. B, the time course of ATF2 phosphorylation by p38 is measured in the presence (□) or absence (▽) of 10 nM MK2. No ATF2 (MK2 only, Δ) and no enzyme (×) are shown as controls. Data is shown ± standard deviation (n = 4).