Differences between CheY6 and classical CheY proteins. (A) Alignment of R. sphaeroides CheY1-6 and E. coli CheY. Blue stars: residues essential for phosphorylation of D57 in E. coli (D12, D13, T87, Y106 and K109). Residues highlighted in yellow in CheY6 show important differences to other CheY homologues. This figure was made using ESPript . (B) RONN  disorder prediction for R. sphaeroides CheY6 showing the disordered C-terminus.