Figure 2

Comparison of model predictions to experimental observations. C. thermocellum iSR432 was used to simulate growth in multiple conditions. Actual and predicted reaction flux rates are shown, and predicted fermentation product production rates are shown as ranges as determined by flux variability analysis (see Methods). For each simulation, the boundary fluxes for cellobiose, acetate, and formate were constrained to match the measured fluxes during (A) chemostat growth on cellobiose and (B) fructose[32], and (C) batch growth on cellobiose[33].