Boundary smoothing. A. Fluorescent image of a chemotaxing cell. B. The closed curve shows the edge of the segmented shape before smoothing; the line segments form the skeleton obtained from this shape. C. Smoothed boundary and resultant skeleton. D. Comparison of the original (grey) and post-boundary-smoothing (black) skeletons. Note that several branches deemed to arise from noise fluctuations in the image have disappeared.