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Figure 5 | BMC Systems Biology

Figure 5

From: Automated characterization of cell shape changes during amoeboid motility by skeletonization

Figure 5

Individual pseudopods analysis. A-C. State and angle dynamics for pseudopods with consistent protrusions (A), consistent retractions (B) and protrusions followed by retractions (C). The movie was obtained courtesy of N. Andrew and R. H. Insall. The images show superimposed cell shapes (from the beginning of each pseudopod) and activity trajectories (red for protrusions, blue for retractions). The numbers represent the time (in seconds) from the beginning of the movie. The corresponding activity profiles relative to the chemoattractant gradient are plotted in D-F. In these plots red and blue dots represent protrusions and retractions, respectively, and the green lines join activities coming from the same pseudopod. G-J. The angle dynamics of all pseudopods superimposed by the directions of cell centroid (light-blue dots). Pseudopods with consistent protrusion pattern, consistent retraction pattern, protrusion followed by retraction pattern are highlighted in frame G-I, respectively. All the short-lived pseudopods are highlighted in J.

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