Protrusion angle distributions for different strains during chemotaxis. Protrusion angle histogram (green) fitted by a Gaussian curve (red line) for vegetative AX3 cells (A) and AX3:tsuA cells (B), both in the gradient of folic acid released from a needle , as well as those for developed AX2 cells (C) and developed AX2:dynhp cells (D), both in the gradient of cAMP released from a needle . The Gaussian fittings yield different values of mean ± STD: -15.5° ± 90.3° for vegetative AX3 cells (A), 0.0° ± 29.1° for developed AX2 cells (C), -2.3° ± 62.8° for developed AX2:dynhp cells (D). The Gaussian fitting for vegetative AX3:tsuA cells does not converge (B). The comparisons of other pseudopod statistics between these strains are summarized in Table 3.