Correlation analysis between pseudopodial activities and protein localization. Scatter plot between activity length (positive length implies a protrusion, negative length a retraction, measured in pixels) and dynacortin-mCherry (number of standard deviations above the mean fluorescence around each cell) for all activities both at the site of the activity (A) and 180° away (B). The correlation coefficient (ρ) for each plot is given. These data represent 3333 activities in 37 cells. C. Histogram for the correlation coefficients for each cell at the site of the activity (red) and 180° away (blue). D. Mean dynacortin-mCherry fluorescence at the sites of protrusions and retractions (error bars are SEM). E-H. Similar plots for myosin-II-GFP. These data represent 9019 activities in 100 cells.