Table 2 Quantities used to describe morphological behaviors.
From: Automated characterization of cell shape changes during amoeboid motility by skeletonization
Classification | Name | Description |
|---|---|---|
Individual pseudopods | ||
   General | Lifetime | The length of time period when the pseudopod has protrusion or retraction activity |
| Â | Number of detected activities | The total number of detected activities during the lifetime |
| Â | Net speed | The net displacement of local membrane during the lifetime divided by the time length |
   State dynamics | Protrusion/retraction ratio | Ratio of protrusion/retraction activities out of all detected activities |
| Â | Protrusion/retraction speed | Total length of protruded/retracted displacement divided by the total length of protrusion/retraction time |
| Â | Protrusion/retraction persistence | The longest running time of consistent protrusions/retractions divided by the lifetime |
| Â | State persistence | The longest running time of consistent state (either protrusion or retraction) divided by the lifetime |
   Angle dynamics | Starting angle | The angle at which the first activity of the pseudopod is detected |
| Â | Mean angle | The angle averaged over all activities in the pseudopod |
| Â | Linear angle changing rate | The slope of the line that fits best to the activity angle changes |
| Â | De-trended angle variance | The variance of activity angles after removal of the linear changing rate |
   Splitting behavior | Origin | The pseudopod is generated newly or by splitting from existing ones |
| Â | Number of splits | How many splits are generated from the pseudopod during its lifetime |
Cell Level | Â | Â |
   Group behavior | Average number of pseudopods | The average number of active pseudopods coexisting on the cell membrane at any given time point |
| Â | Ratio of pseudopods from splitting | Number of pseudopods generated from splitting divided by the total number of pseudopods |
| Â | Average splitting rate | Total number of splits occurred divided by the time length of the period of movement |
   Averaged behavior | Average protrusion/retraction persistence | Computed by averaging the corresponding quantities for individual pseudopods over all pseudopods in the cell. |
| Â | Average state persistence | Â |
| Â | Average protrusion/retraction speed | Â |
| Â | Average net speed | Â |