Figure 5
From: Building and analyzing protein interactome networks by cross-species comparisons
Western blots of co-immunoprecipitations (coIP) of predicted and known interactions. CoIP was conducted with anti-FLAG, and membranes were probed with anti-HA, stripped, and probed with anti-FLAG. Bands indicate a presence of tagged protein. "Input" is total protein before coIP, while "FLAG-IP" is protein present after coIP. If a band is present in both the input and FLAG-IP lanes, it indicates that the protein has been pulled down with the anti-FLAG antibody. When the protein in question is tagged with HA instead of FLAG, this indicates an interaction between the FLAG-tagged protein and the HA-tagged protein. InteroScores are given in parentheses: PPI between LSm7 and CG6610 (3.27), LSm7 and SmD3 (1.27), CG2021 and CG17768 (3.27), CG9344 and CG6610 (3.27), LSm7 and CG17768 (3.27), and CG12703 and spn-A (1.27) were positive controls of known PPI. Predicted PPI that were confirmed to interact were: CG10418 and CG6610 (1.39), LSm7 and CG2021 (1.69), LSm7 and CG9344 (1.39), CG9344 and CG2021 (2.69), RfC4 and RfC38 (2.64), RfC4 and CG8142 (3.27), DNApol-alpha50 and DNApol-alpha60 (1.39), MED26 and MED4 (1.18), ix and MED28 (1.18), CG5325 and CG5525 (1.39), MED17 and MED26 (1.18). Those that were not confirmed were: CG10418 and CG17768 (1.77), CG10418 and SmD3 (1.53), dup and CG6905 (1.12), and MED6 and MED26 (1.18).