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Table 2 Simulation and experimental results for the case study of TNF-α-induced NFκB pathway.

From: A formal model for analyzing drug combination effects and its application in TNF-α-induced NFκB pathway

Inhibitor A Inhibitor B Synergism Assessment Factor (Simulation) Predicted interaction Synergism Assessment Factor (Experiment)
   simplified system original system   
Geldanamycin Aldehyde -0.0632 -0.2678 Synergism -0.0167
Geldanamycin PS-1145 -0.1248 -0.2555 Synergism -0.1878
PS-1145 Aldehyde -0.1754 -0.1754 Synergism -0.3931
  1. Here we consider the synergism assessment factors of the three combinations in the simplified and original system. The sign of synergism assessment factor indicates whether the drug combination generates synergistic effect or not. If the sign is negative, it is synergistic effect. Otherwise, it is antagonistic effect. In the experiment, ICAM-1 expression is considered as the measurement readout of the efficacy of drugs or drug combinations. We also evaluate the effects of drug combination by calculating synergism assessment factor (see Eq. 3). The negative values represent synergism since we rewrite calculation formula of Bliss independence model according to survival ratio rather than inhibition ratio.