Figure 5

Validation of a Direct interaction between miR-141 and SEMA6A. Results of in-vitro experiment on RCC cell line CRL-1611 transfected either with pre-miR-141 or a control pre-miR using either Fugene (Fu) or Hyfect (Hy). RNA was extracted after 48 hours and the expression of SEMA6A was measured using semi-quantitative RT-PCR. There is clear reduction in mRNA of SEMA6A upon introduction of miR-141 by either transfection method in these cells.