OptORF: Optimal metabolic and regulatory perturbations for metabolic engineering of microbial strains

BMC Systems Biology

Table 1 OptORF formulation

maximize	biochemical production
subject to
	maximize	cellular growth
	subject to	steady-state mass balance
		enzyme capacity
		thermodynamics
		reaction deletions
	GPR associations
	transcriptional regulations
	gene deletions and overexpressions
	limited number of gene deletions
	limited number of gene overexpressions

ISSN: 1752-0509