Table 1 OptORF formulation
| maximize | biochemical production | |
|---|---|---|
| subject to | ||
| maximize | cellular growth | |
| subject to | steady-state mass balance | |
| enzyme capacity | ||
| thermodynamics | ||
| reaction deletions | ||
| GPR associations | ||
| transcriptional regulations | ||
| gene deletions and overexpressions | ||
| limited number of gene deletions | ||
| limited number of gene overexpressions | ||