maximize | biochemical production | |
---|---|---|
subject to | Â | Â |
 | maximize | cellular growth |
 | subject to | steady-state mass balance |
 |  | enzyme capacity |
 |  | thermodynamics |
 |  | reaction deletions |
 | GPR associations | |
 | transcriptional regulations | |
 | gene deletions and overexpressions | |
 | limited number of gene deletions | |
 | limited number of gene overexpressions |