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Table 3 Gene deletion and overexpression strategies for ethanol production in E. coli.

From: OptORF: Optimal metabolic and regulatory perturbations for metabolic engineering of microbial strains

Deleted Genes

Overexpressed Genes

Growth Rate (hr-1)

Ethanol Yield (%)

fnr

pflB

tdcE

pgi

 

edd

0.225

86.2

fnr

pflB

tdcE

pgi

ptsH

edd       fbp

0.182

90.4

fnr

pflB

tdcE

tpiA

 

edd

0.235

90.5

fnr

pflB

tdcE

tpiA

gdhA

edd

0.214

91.4

arcA

pta

eutD

tpiA

ptsH

edd

0.192

91.6

  1. In addition to gene deletions, overexpressed genes are identified to further improve ethanol production. The maximum number of overexpressed genes was limited to two for all cases, and strategies including less than four gene deletions are not shown due to the negligible increase in ethanol yields by gene overexpression.