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Table 1 Reaction stoichiometry of the dynamic network model.

From: Dynamics and Control of the Central Carbon Metabolism in Hepatoma Cells

   Stoichiometry
Reaction number Reaction name Substrates Products
r1 glucokinase GLCin + ATPin G6Pin + ADPin + Hnb
r2 glucose-6-phosphate isomerase G6Pin F6Pin
r3 phosphofructokinase F6Pin + ATPin F16Pin + ADPin + Hnb
r4 fructose-bisphosphate aldolase F16Pin DHAPin + GAPin
r5 triose-phosphate isomerase DHAPin GAPin
r6 glyceraldehyde-3-phosphate dehydrogenase GAPin + Pnb + NADin 13PGin + NADHin + Hnb
r7 phosphoglycerate kinase 13PGin + ADPin + Hnb G3Pin + ATPin
r8 phosphoglycerate mutase G3Pin G2Pin
r9 pyruvate kinase PEPin + ADPin + Hnb PYRin + ATPin
r10 glucose-6-phosphate dehydrogenase G6Pin + NADPin GL6Pin + NADPHin + Hnb
r11 6-phosphogluconolactonase GL6Pin + H2Onb 6GPin + Hnb
r12 phosphogluconate dehydrogenase 6GPin + NADPin RIBU5Pin + CO2nb + NADPHin
r13 ribose-5-phosphate isomerase RIBU5Pin RIBO5Pin
r14 ribulose-phosphate 3-epimerase RIBU5Pin XYL5Pin
r15 transketolase RIBO5Pin + XYL5Pin GAPin + S7Pin
r16 transketolase XYL5Pin + E4Pin GAPin + F6Pin
r17 phosphopyruvate hydratase G2Pin PEPin + H2Onb + Hnb
r18 lactate dehydrogenase PYRin + NADHin + Hnb LACin + NADin
r19 adenosinetriphosphatase ATPin + H2Onb ADPin + Pnb + Hnb
r20 alanine transaminase ALAin + AKGin PYRin + GLUin
r21 phosphoglucomutase G6Pin G1Pin
r22 nadph consumption NADPHin + Anb NADPin + AHnb
r23 glycogen synthesis UTPnb + G1Pin UDPnb + GLYGnb + Hnb + PPnb
r24 transaldolase S7Pin + GAPin F6Pin + E4Pin
r25 adenylate kinase ATPin + AMPin 2·ADPin
r26 glycerol synthesis DHAPin GLYCEROLnb
r27 nucleotide synthesis RIBO5Pin + ATPin PRPPin + AMPin + Hnb
r28 serine synthesis G3Pin + NADin + GLUin + H2Onb SERin + Pnb + NADHin + 2·Hnb + AKGin
r29 citrate synthase OACin + ACCOAin + H2Onb CITin + COAin + Hnb
r30 aconitate hydratase CITin CISACin + H2Onb
r31 aconitate hydratase CISACin + H2Onb ISOCITin
r32 isocitrate dehydrogenase ISOCITin + NADin AKGin + CO2nb + NADHin
r33 succinate-CoA ligase SUCCOAin + Pnb + ADPin SUCin + COAnb + ATPin
r34 fumarate hydratase FUMin + H2Onb MALin
r35 malate dehydrogenase MALin + NADin OACin + NADHin + Hnb
r36 pyruvate dehydrogenase complex PYRin + NADin + COAnb CO2nb + NADHin + ACCOAin
r37 alpha-ketoglutarate dehydrogenase complex AKGin + NADin + COAnb SUCCOAin + CO2nb + NADHin
r38 pyruvate synthesis PYRnb PYRin
r39 valine leucine isoleucine metabolism ISOVALMETnb SUCCOAin
r40 succinate dehydrogenase SUCin + 0.6·NADin +0.2·O2nb FUMin + 0.6·NADHin + 0.6·Hnb + 0.4·H2Oin
r41 oxidative phosphorylation NADHin + 0.5·O2nb + 3.5·Hnb + 2.5·ADPin + 2.5·Pnb NADin + 3.5·H2Onb + 2.5·ATPin
r42 alpha-ketoglutarate synthesis AKGnb AKGin
r43 malic enzyme MALin + NADPin PYRin + CO2nb + NADPHin
r44 glutamate dehydrogenase GLUin + H2Onb + NADin AKGin + NH4nb + NADHin + Hnb
r45 lactate transport LACin LACex
r46 pyruvate transport PYRin PYRex
r47 glucose transport GLCin GLCex
r48 alanine transport ALAex ALAin
r49 serine transport SERin SERin
  1. The subscripts 'ex', 'in', and 'nb' denote extracellular, intracellular, and non-balanced metabolites, respectively.
  2. The Phosphoenolpyruvate carboxykinase enzyme was found to be inactive in the reference state [7], and, thus, it was not included in the dynamic network model.