Figure 7

Correction of reporter concentrations for different mRNA and protein half-lives. (a) Original (blue line) and corrected (green line) GFP concentration profile. The correction accounts for the systematic bias γ _n /γ _m = 0.54 and γ _q /γ _p = 1.7. Both profiles are normalized with respect to the peak in mid-exponential phase. The 95% confidence bands are shown as dashed lines and the Western blot measurements are taken from Figure 5. (b) Idem for luciferase and Fis, γ _n /γ _m = 0.59 and γ _q /γ _p = 1.8. (c) Robustness of computed protein concentration to systematic errors caused by differences in half-lives of products of gfp and fis. The figure shows the curves for γ _n /γ _m and γ _q /γ _p values equal to 0.25, 1, and 4. The clearly separated curves correspond to different values of γ _q /γ _p . Within each set, the different ratios of the mRNA half-lives have very little effect. (d) Idem for lux.