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Table 2 Predicted essential genes and potential drug targets.

From: Identifying essential genes in bacterial metabolic networks with machine learning methods

ORF Gene Symbol EC Enzyme Evidence
a) Intersection of our predictions with the experimental screen
STM0123 murE 6.3.2.13 UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase **
STM0128 murG 2.4.1.227 N-acetylglucosaminyl transferase *
STM0129 murC 6.3.2.8 UDP-N-acetylmuramate-L-alanine ligase **
STM0154 lpdA 1.8.1.4 Dihydrolipoamide dehydrogenase  
STM0218 pyrH 2.7.4.22 Uridylate kinase *
STM0221 uppS 2.5.1.31 Undecaprenyl pyrophosphate synthase **
STM0222 cdsA 2.7.7.41 CDP-diglyceride synthase  
STM0228 lpxA 2.3.1.129 UDP-N-acetylglucosamine acyltransferase  
STM0232 accA 6.4.1.2 Acetyl-CoA carboxylase **
STM0489 hemH 4.99.1.1 Ferrochelatase *
STM0535 lpxH   UDP-2,3-diacylglucosamine hydrolase  
STM0542 folD 1.5.1.5, 3.5.4.9 Bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase  
STM0988 kdsB 2.7.7.38 CTP:CMP-KDO cytidylyltransferase *
STM1194 fabD 2.3.1.39 Acyl carrier protein S-malonyltransferase *
STM1195 fabG 1.1.1.100 3-ketoacyl-(acyl-carrier-protein) reductase **
STM1200 tmk 2.7.4.9 Thymidylate kinase  
STM1700 fabI 1.3.1.10 Enoyl-(acyl carrier protein) reductase  
STM2483 dapE 3.5.1.18 Succinyl-diaminopimelate desuccinylase  
STM2652 pssA 2.7.8.8 Phosphatidylserine synthase *
STM3090 metK 2.5.1.6   
STM3415 rpoA 2.7.7.6 DNA-directed RNA polymerase subunit alpha  
STM3724 kdtA   3-deoxy-D-manno-octulosonic-acid transferase *
STM3730 dfp 4.1.1.36 Pantothenate kinase **
STM3912 rep 3.6.1.- ATP-dependent DNA helicase Rep *
STM3978 yigC   3-octaprenyl-4-hydroxybenzoate decarboxylase  
STM4153 rpoB 2.7.7.6 DNA-directed RNA polymerase subunit beta *
STM4154 rpoC 2.7.7.6 DNA-directed RNA polymerase subunit beta'  
b) Predictions for the non-mevalonate pathway
STM0049 ispH, lytB 1.17.1.2 4-hydroxy-3-methylbut-2-enyl diphosphate reductase *
STM0220 dxr 1.1.1.267 1-deoxy-D-xylulose 5-phosphate reductoisomerase *
STM0422 dxs 2.2.1.7 1-deoxy-D-xylulose-5-phosphate synthase **
STM0423 ispA 2.5.1.10 geranyltranstransferase *
STM1779 ispE, ipk 2.7.1.149 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase **
STM2523 ispG, gcpE 1.17.7.1 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase *
STM2929 ispF 4.6.1.12 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase *
STM2930 ispD 2.7.7.60 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase *
  1. ** clear evidence to be a drug target
  2. * reasonable evidence to serve as a good drug target