The estimated translational efficiencies of the modified RDS sequences for the MS2 coat gene. The estimated translational efficiencies (A), probabilities of ribosome-bound mRNA (B), overall RDS-exposure probabilities (C), RRS-hybridization energies (D), and expression levels of MS2 coat genes harboring modified RDS sequences as well. As the expression levels were normalized, the estimated translational efficiencies were also normalized for comparison. The estimated translational efficiency had a high correlation (R2 = 0.77) to the gene expression level when linearly regressed. Consistent with the experimental study, the properties of the MS2 coat genes were estimated at 42°C.