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Figure 5 | BMC Systems Biology

Figure 5

From: Mathematical modeling of translation initiation for the estimation of its efficiency to computationally design mRNA sequences with desired expression levels in prokaryotes

Figure 5

The constructed plasmids and designed synthetic RDS sequences. (A) Schematic of the plasmid harboring the luxR gene with the designed RDS sequences. Based on a genetic algorithm, the 10 nucleotides upstream of the start codon were modified to create synthetic RDS sequences with specific translational efficiencies. For measurement of LuxR protein expression, the lacZ╬▒ gene was fused to the luxR gene. (B) The designed synthetic RDS sequences are listed; the RRS sequences are indicated in bold. For sRDS21 and 22 they have the same designed spacer sequence but their SD sequences were modified further.

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