The designed DsRed2 transcripts and measurement of red fluorescence intensities. (A) The transcript sequences, including the designed RDS nucleotides, of the DsRed2 gene. The RRS sequences are in bold and the RDS regions are in upper-case letters. (B) The predicted RDS secondary structures of the designed DsRed2 mRNA sequences. The RDS nucleotides are in upper-case letters. (C) The expression levels of the designed DsRed2 mRNAs in control (null plasmid), DsRed2-L (designed for low expression), and DsRed2-H (designed for over-expression) experiments. The cells containing the designed DsRed2 genes are shown over the plot.