Expression trend of lineage-specific genes during differentiation of CB CD34+ and miRNA microarray data confirmation. (A) The reliability of gene microarray results was inspected by comparing the variations in signal of well-known lineage-specific genes for each unilineage cultures. The expression of the selected genes was concordant with the expected protein modulation during differentiation/maturation. (B) Real-time reverse-transcription polymerase chain reaction of six randomly chosen differentially expressed miRNAs in erythroid, megakaryocytic granulocytic, and monocytic unilineage cultures. qRT-PCR results are compared to miRNA microarray derived values.