Figure 3
E. coli K-12 MG1655 central carbon metabolism flux, protein and mRNA dynamics with rising specific growth rate in three A-stat cultivations. μ, specific growth rate (h-1). A. Flux or expression fold change (log2) is calculated for each μ compared to μ = 0.10 h-1; grey box depicts missing value; Acs flux was switched to Pta after μ = 0.31 h-1 in MFA since acetate excretion exceeds its production accompanying biosynthesis. Protein and mRNA data taken from Valgepea et al. [11]. Error bars represent standard deviation of triplicate A-stat experiments. B. Selected glycolysis, TCA cycle, PPP, gluconeogenesis and acetate related fluxes.