Reproducibility of Protein Activities Across Simulated Replicates. (A) The heatmap shows the coefficient of variation for protein activity (columns) following different treatments (rows). The color intensity increases with decreasing reproducibility. The nine treatments shown in the rows are (i) EGF, TNFα, IGF-1, and INS combined; (ii) TNFα and INS; (iii) TNFα and IGF-1; (iv) TNFα and EGF; (v) EGF only; (vi) IGF-1 only; (vii) INS only; (viii) TNFα only; (ix) and no active extracellular ligands (Control). Each cell in the heatmap represents the coefficient of variation for the activity of one protein across 100 replicates with 1000 cells/replicate (a total of 100,000 Boolean network simulations). (B) The distribution of CV across each treatment condition. (C) The distribution of CV across each protein.