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Table 1 Plasmids and strains used in this study

From: Negative auto-regulation increases the input dynamic-range of the arabinose system of Escherichia coli

Plasmids Description Source
pUA66 sc101 ori, promoterless version of the GFP reporter plasmid, (kanR). [40]
P araBAD reporter GFP reporter plasmid for the araBAD promoter (P araBAD in pUA66), (kanR). [40]
P araC reporter GFP reporter plasmid for the araC promoter, (P araC in pUA66), (kanR). [40]
pZE11 Control plasmid: colE1 ori, P LtetO-1 promoter, (ampR) [45]. This study
pZE11-araC araC controlled by the tet promoter on pZE11, (ampR). This study
Strains   
U423 MG1655z1: MG1655 (F- lambda- ilvG- rfb-50 rph-1) with chromosomal tetR, (specR). This study
U424 U423 +pZE11 +P araBAD reporter plasmid (specR, ampR, kanR). This study
U425 U423 with ΔaraC chromosomal deletion (specR). This study
U426 U425 +pZE11-araC +P araBAD reporter plasmid (specR, ampR, kanR). This study
U427 U423 +pZE11 +pUA66 (specR, ampR, kanR). This study
U428 MG1655 (F- lambda- ilvG- rfb-50 rph-1) + P araC reporter plasmid (kanR). [40]
U429 MG1655 (F- lambda- ilvG- rfb-50 rph-1) + P araBAD reporter plasmid (kanR). [40]
U66 MG1655 (F- lambda- ilvG- rfb-50 rph-1) + pUA66 (kanR). [40]