Table 1 Plasmids and strains used in this study

pUA66

sc101 ori, promoterless version of the GFP reporter plasmid, (kan^R).

[40]

P _araBAD reporter

GFP reporter plasmid for the araBAD promoter (P _araBAD in pUA66), (kan^R).

[40]

P _araC reporter

GFP reporter plasmid for the araC promoter, (P _araC in pUA66), (kan^R).

[40]

pZE11

Control plasmid: colE1 ori, P _LtetO-1 promoter, (amp^R) [45].

This study

pZE11-araC

araC controlled by the tet promoter on pZE11, (amp^R).

This study

Strains

U423

MG1655z1: MG1655 (F- lambda- ilvG- rfb-50 rph-1) with chromosomal tetR, (spec^R).

This study

U424

U423 +pZE11 +P _araBAD reporter plasmid (spec^R, amp^R, kan^R).

This study

U425

U423 with ΔaraC chromosomal deletion (spec^R).

This study

U426

U425 +pZE11-araC +P _araBAD reporter plasmid (spec^R, amp^R, kan^R).

This study

U427

U423 +pZE11 +pUA66 (spec^R, amp^R, kan^R).

This study

U428

MG1655 (F- lambda- ilvG- rfb-50 rph-1) + P _araC reporter plasmid (kan^R).

[40]

U429

MG1655 (F- lambda- ilvG- rfb-50 rph-1) + P _araBAD reporter plasmid (kan^R).

[40]

U66

MG1655 (F- lambda- ilvG- rfb-50 rph-1) + pUA66 (kan^R).

[40]