Figure 3From: A retrosynthetic biology approach to metabolic pathway design for therapeutic productionFlowchart for ranking pathways. In order to rank pathways, each reaction r = 1 ... N in the enumerated pathways p1 ... p M is first tested for thermodynamical feasibility; enzyme candidates are subsequently tested for performance and homogeneity so that the one with the lowest cost is selected; the cost of toxicity of each reaction product is then added; finally, the nominal flux is estimated for the overall pathway.Back to article page