A retrosynthetic biology approach to metabolic pathway design for therapeutic production

BMC Systems Biology

Table 4 Ranked pathways for biosynthesis of taxol in S. cerevisiae

EC number	product	cost	ρ ₁	ρ ₂
2.3.1.- *	C07394	1.34	X	X
2.3.1.167	C11900	1.17	X	X
2.3.1.166	C11700	1.07	X	X
1.14.14.1*	C11899	1.78	X	X
1.14.13.76	C11898	5.64	-	X
1.14.13.77	C11897	5.62	X	-
2.3.1.162	C11896	1.06	-	X
1.14.99.37	C11895	5.62	X	X
4.2.3.17	C11894	1.28	X	X
2.5.1.29	C00353	0.69	X	X
		v_c(ρ)	0.094	0.093
		W(ρ)	18.64	19.72

Each row corresponds to the insertion of one enzyme in the pathway in order to produce the given intermediate product in the second column. The estimated cost is given in the third column 3. The last two rows provide the estimate of maximum flux v_c(ρ) for the pathway, and the total cost W(ρ) according to Equation 3. C07394: Paclitaxel; C11900: Baccatin; C11700: 10-Deacetylbaccatin; C11899: 10-Deacetyl-2-debenzoylbaccatin; C11898: Taxa-4(20),11(12)-dien-5α-acetoxy-10β-ol; C11897: Taxa-4(20),11(12)-dien-5α,13α-diol; C11896: Taxa-4(20),11(12)-dien-5α-yl; C11895: Taxa-4(20),11(12)-dien-5α-ol; C11894: Taxa-4(5),11(12)-diene; C00353: Geranylgeranyl. Starred EC numbers correspond to putative enzymes.

ISSN: 1752-0509