Fluxomers: a new approach for 13C metabolic flux analysis

BMC Systems Biology

Table 5 Metabolic fluxes comparison for wild-type and mutant C. glutamicum

	Wildtype					Mutant
	Becker	OpenFLUX	FIA			Becker	OpenFLUX	FIA
			const.	meas.	ratios			const.	meas.
Glucose 6-phosphate isomerase	49.8	51.2	51.9	52.0	51.5	41.6	40.4	42.1	42.5
Glucose 6-phosphate dehydrogenase	46.8	45.0	44.7	44.7	45.1	56.2	57.5	55.7	55.1
Transaldolase	14	13.4	13.3	13.3	13.4	17.5	17.7	17.3	17.0
Transketolase 1	14	13.4	13.3	13.3	13.4	17.5	17.7	17.3	17.0
Transketolase 2	11.9	11.3	11.2	11.2	11.3	15.8	16.4	15.6	15.4
Glyceraldehyde 3-phosphate dehydrogenase	157.5	158.0	158.2	158.6	158.0	160.8	161.0	161.0	160.5
Pyruvate kinase	147.3	148.0	147.8	148.2	147.6	152.6	152.0	152.5	152.0
Pyruvate dehydrogenase	77.5	75.8	74.8	74.9	74.9	87.5	85.2	85.1	79.7
Pyruvate carboxylase - carboxykinase	34.4	35.8	35.9	36.1	35.8	31.5	32.4	32.5	34.9
Citrate synthase	52.5	50.8	49.6	49.7	49.9	67.7	65.4	65.3	58.9
Isocitrate dehydrogenase	52.5	50.8	49.6	49.7	49.9	67.7	65.4	65.3	58.9
Oxoglutarate dehydrogenase	41.2	39.4	38.2	38.3	38.5	59.9	57.6	57.5	50.7
Aspartokinase	11.2	11.2	11.2	11.4	11.2	14.2	14.2	14.2	15.9

Estimated metabolic fluxes values for the different approaches - the ad-hoc simulation results from Becker et al. [18], the OpenFLUX results [15], and the FIA results for its various simulated scenarios (measured fluxes used as constants, as measurements, and when using ratios of non-normalized data.)

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