Comparison of i CM925 simulations with experimental data. Model and experimental values for product fluxes, uptake fluxes and growth rates represent conditions for the 35°C fermentation. Error bars indicate the observed experimental range and diamonds represent the various simulation results. (A) shows the simulation results for the case where only acetate and glucose uptake rates are constrained. (B) shows the case where these uptake rates, as well as butanol, acetone, and butyrate formation rates are constrained. In (B), the blue diamonds represent the case where non-growth associated ATP maintenance is zero and the yellow diamonds represent the case where the non-growth associated ATP maintenance is 8.5 mmol/gDW/hr.