Figure 8

Effects of ternary complex binding rate (Model 3). This effect was analysed under circumstances where scanning rate was decreased by 3-fold (A and B), and 5'-initiation rate was increased to 3-fold (C and D). GCN4 mRNA translation was simulated at each specific ternary complex binding rate for 60 min. The polysome sizes for the two sections were averaged over 10-60 min. 50 such replicates were averaged to determine the polysome sizes (A and C) for each ternary complex binding rate. The translation rate of uORF1 and GCN4 was also monitored. Subsequently, we calculated the ratio between GCN4 translation and uORF1 translation for each ternary complex binding rate in the same way of averaging (B and D).