Figure 9From: Analysing GCN4 translational control in yeast by stochastic chemical kinetics modelling and simulationEffects of histidine codons translation rates (Model 3). This effect was analysed under circumstances where scanning rate was decreased by 3-fold (A and B), and 5'-initiation rate was increased to 3-fold (C and D). To ensure a constant GCN4 reinitiation rate, Ternary complex binding rate was set to maintain its derepressing ratio with the scanning rate under each condition (10 and 30 nt/s, respectively). Other parameters were the same as in Figure 6. The two histidine codon rates were changed to the same degree, from 0.0001-to 1-fold relative to its repressing value (i.e. relative codon rate). Simulations results were obtained and averaged in the same way as Figure 8. 5'-and 3'-polysome sizes (A, C) and uORF1 to GCN4 translation rate ratio (B, D) were plotted against relative codon rate.Back to article page