Figure 2

Parameter portraits of the systemic behavior of a computational model of the E. coli thioredoxin system. Changes in the thioredoxin reductase (TR) concentration affected the fluxes of thioredoxin-dependent reactions (A) and the steady state thioredoxin (blue, •) and oxidized thioredoxin concentrations (red, -) (B) in the model. Similarly, changes in the protein disulfide (C), PAPS (D), methionine sulfoxide (E) and hydrogen peroxide (F) concentrations also affected the fluxes through the system. The PAPS reductase reaction was modeled with mass action kinetics (Figures 2 A-C, E-F) or with ping-pong kinetics (Figure 2D) (see Methods for details). The fluxes shown are thioredoxin reductase (black, •••), protein disulfide reduction (red, ---), methionine sulfoxide reductase (blue, •••), PAPS reductase (green, -•-) and Tpx (magenta, -). Parameters are summarized in Table 1. This figure was generated using 'Logic_Figure 2_and Figure 4.psc' (Additional file 1) and 'Logic_Figure 2 D.psc' (Additional file 2). SBML versions of these models, 'Logic_Figure 2_and Figure 4.xml' (Additional file 5) and 'Logic_Figure 2 D.xml' (Additional file 6), are provided as well.