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Table 2 Kinetic parameters and metabolite concentrations used in core models of the E. coli thioredoxin system.

From: The logic of kinetic regulation in the thioredoxin system

 

Value

Metabolites

(μ M)

NADPH

1

NADP

1

TrxSH

0.5

TrxSS

0.5

PSS

1

PSH

1

RSS

1

RSH

1

Thioredoxin

 

reductase

 

[TR]

1 μ M

k cat

1 s-1

K nadph

1 μ M

K trxss

1 μ M

PSS reduction

 

k 2

1 μ M-1s-1

RSS reduction

 

k 3

1 μ M-1s-1

  1. Core models of the thioredoxin system based on Schemes I and II were created using Michaelis-Menten kinetics for thioredoxin reductase and mass action kinetics for the reduction of PSS and RSS by thioredoxin. To simplify the analysis, all reaction parameters in these models were set to one. The parameters listed in this table were used in the generation of Figures 3 and 5.