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Table 2 Model-based predictions and experimental data on net CO2 release, biomass yield and RubisCO flux for photoheterotrophic growth of PNSB on different substrates.

From: Metabolic network modeling of redox balancing and biohydrogen production in purple nonsulfur bacteria

  Succinate Malate Acetate Fructose Propionate Hydrogen (autotroph)
Net CO2 release Scenario S1/S2 Scenario S1/S2 Scenario S1/S3/S4/S5 Scenario S1/S2 Scenario S1/S2 Scenario S1/S2
calculated [mmol CO2/mmol substrate] 0.72 1.19 0.13 0.38 -0.28 -0.47
([mmol CO2/C in substrate]) (0.181) (0.300) (0.064) (0.064) (-0.094) (∞)
measured [mmol CO2/mmol substrate] 0.75 [37] 1.15 [37] 0.11 [10]   -0.23 [37]  
  0.70 [36] 1.22 [36] 0.25 [37]    
    0.17 [36]    
Biomass yield Y X/S Scenario S1/S2 Scenario S1/S2 Scenario S1/S3/S4/S5 Scenario S1/S2 Scenario S1/S2 Scenario S1/S2
calculated [gDCW/mmol substrate] 0.072 0.062 0.041 0.124 0.072 0.010
calculated [gDCW/g substrate] 0.610 0.463 0.683 0.688 0.973 5.000
measured [gDCW/g substrate]   0.476 [52] 0.660 [44]    
    0.650[10]    
RubisCO flux Scenario S2 Scenario S2 Scenario S3/S4/S5 Scenario S2 Scenario S2 Scenario S2
Calculated minimal RubisCO flux [mmol/mmol substrate] 0.73 0.20 S3: 0.22 0.40 0.73 0.53
    S4: 0.22    
    S5: 0    
[mmol/mmol C in substrate] (0.184) (0.05) S3: 0.11 (0.067) (0.245) (∞)
    S4: 0.11    
    S5: 0    
Measured [mmol/mmol substrate]    S3: 0.29[10]    
  1. The data taken from references [36] and [37] are averaged values over replicated experiments.