Figure 3

Positive regulation of pou6f1 to tmem59 is evident in C17.2 NSCs. (A, B) gray photos were captured in white light. (C, D)POU6F1-EGFP (green) was over-expressed in the nucleus of C17.2 neural stem cells. Photography was captured at 36-hours after transfection of pEGFP-N2-Pou6f1 plasmid. (E) Real-time PCR showed that tmem59 is up-regulated by over-expression of pou6f1 (normalized to β-actin). N = 3, **P < 0.01. OE: over-expression of POU6F1-EGFP; Con: control group transfected with pEGFP-N2.