Kinase inhibitors can produce off-target effects and activate linked pathways by retroactivity

BMC Systems Biology

Table 1 The parameter space of each network consisted of a set of non-dimensional parameters, each with a minimum and maximum allowed value.

	default range
parameter	minimum	maximum	description
E _i	0.01	100	total kinase to total substrate ratio
E' _i	0.01	100	total phosphatase to total substrate ratio
K _i	0.01	100	normalized K_m of kinase reaction
K' _i	0.01	100	normalized K_m of phosphatase reaction
P _i	0.1	10	ratio of the kinase reaction V_max to the phosphatase reaction V_max
K _B	0.01	100	normalized drug disassociation constant

Each cycle i consisted of 5 dimensionless parameters: E_i, E'_i, K_i, K'_i, and P_i. A final parameter, K_B, applied to the targeted inhibitor. Randomly selected dimensionless parameter values could not exceed the default ranges listed for each parameter type.

ISSN: 1752-0509