The n = 3 network's restricted parameter space produced 73.9% off-target effects. The n = 5 and n = 7 vertical case networks produced 27.9% and 13.5% off-target effects, respectively, using the same parameter space. In the lateral case the drop was more dramatic with the n = 5 and n = 7 networks producing 6% and 0% off-target effects, respectively. Parameter values used in cycles 4, 5, 6 or 7 were homogenous with cycle 3. All percentages are out of 5000 randomly selected parameter sets using the parameter space depicted in Figure 3I.