n

Off Target Effects


3

73.9


vertical

lateral

5

27.9

6.0

7

13.5

0.0

 The n = 3 network's restricted parameter space produced 73.9% offtarget effects. The n = 5 and n = 7 vertical case networks produced 27.9% and 13.5% offtarget effects, respectively, using the same parameter space. In the lateral case the drop was more dramatic with the n = 5 and n = 7 networks producing 6% and 0% offtarget effects, respectively. Parameter values used in cycles 4, 5, 6 or 7 were homogenous with cycle 3. All percentages are out of 5000 randomly selected parameter sets using the parameter space depicted in Figure 3I.