Table 1 List of primers used in RT-PCR reactions.
Transcript amplified | Primer sequence (F 5'to 3'; R 5'to 3') | Product length | Tm (°C) | Cycle # |
|---|---|---|---|---|
PK (pyruvate kinase) | F: AGTCGGAGGTGGAAATTGTG R. AGGTCCACCTCAGTGTTTGG | 267 bp | 60 | 45 |
HK (hexokinase) | F: CAGGGTCTGAGCAAGGAGAC R: GCTTCCTTCAGCAAGGTGAC | 430 bp | 60 | 45 |
PFK(phosphofructokinase) | F: CACCATCAGCAACAATGTCC R: AGTCGTGGATGTTGAAAGGG | 242 bp | 60 | 40 |
GAPDH (Glyceraldehydephosphate dehydrogenase) | F: TCG GAG TCA ACG GAT TTG G R: GCA TTG CTG ATG ATC TTG AGG | 500 bp | 50 | 30 |
CS (citrate synthase) | F: AAGGCTAAAGGTGGGGAAGA R: CCATTCATAGCTGCTGCAAA | 565 bp | 54 | 35 |
Beta-actin | F: GGCTTTAGGAGCTTGACAATACTG R: GCATTGGTCACCTTTAGATGGA | 511 bp | 60 | 30 |