Table 2 Data sets analyzed by RCR for assessment and augmentation of the Cellular Stress Network model
From: A computable cellular stress network model for non-diseased pulmonary and cardiovascular tissue
Data Set | Hyperoxia | HOCl | OxPAPC | Hypoxia |
|---|---|---|---|---|
Data Set ID | GSE495 | GSE15457 | GSE20060 | GSE11341 |
PubMed ID | N/A | 19376150 | 20170901 | 18469115 |
Perturbation | 100% O 2 | 1.4 mM hypochlorous acid | 40 μg/ml oxidized phospholipid | 1% O 2 |
Tissue/Cells | Whole lung | RAW 267.4 cell line | Aortic endothelial cells | Lung microvascular endothelial cells |
In vivo/in vitro | In vivo | In vitro | In vitro | In vitro |
Species | Mouse | Mouse | Human | Human |
Timepoint | 48 h | 6 h | 4 h | 48 h |
Control | 0 h | 6 h untreated | 4 h untreated | 0 h |
Platform | Affymetrix U74v2 | Affymetrix 430_2 | Affymetrix HGU133A | Affymetrix HGU133A |
# State Changes | 1122 | 1618 | 185 | 639 |
Measured outcome(s) | None | Cell viability, RT-PCR, and Western blot analysis of Nrf2 and Nrf2 target genes | qRT-PCR for HMOX1, GJA5 | Hypoxia-induced marker genes; Scratch wound assay |