Figure 9

BAE cell migration towards VEGF and VEGFf. BAECs were seeded on the upper membrane of transwell cell migration chambers. VEGF and VEGFf were added to the lower chamber in various ratios, with the total amount of VEGF/VEGFf combination or alone being 0.45 nM. The cells were incubated for 2 h at 37°C. Migrated cells were fixed, stained with propidium iodide and images collected by a fluorescent microscope (100 × magnification). Each representative image showed one sixth of the field and were used for quantification. The average cell number migrated ± SEM of 12 fields per condition; n = 3. ANOVA followed by the multi-comparison t-tests revealed significant differences between all groups except the no VEGF "Control" and the all VEGFf "0:1.0" conditions.