Figure 5

Avidity model predicts a bimodal distribution of PPDK subunit phosphorylation states. Subunit state configurations were evaluated using a model of spatial arrangements of PPDK subunits. Presented is the steady-state fraction (see Methods) of each configuration of the PPDK tetramer. We focused only on configurations of either PPDK₁ (marked in white) or PPDK₂ (marked in black) subunits. Similar results hold for configurations where one or more subunits are in the PPDK₀ state (see additional file 2). Model parameters are: V_p = V_k = 0.2 [koff1], kon2 = 200 [koff2] and total PPDK/total RP = 100 [11, 13, 33].