EFM analysis of the balance between dissimilation and assimilation in EMCP and ICL variants. The biomass-forming EFMs were calculated and sorted according to the biomass yield (from bottom to top, blue color scale). For each EFM, the flux through the main dissimilatory processes (see text for details) were extracted and plotted separately. Fluxes were expressed relative to the rate of methanol uptake, and were plotted using a colour-scale (black to green). First lane: cytoplasmic FDH, fdh(c); second lane: periplasmic FDH, fdh(p); third lane: TCA oxidation of acetyl-CoA, TCAc; fourth lane: other dissimilation process. A: EFMs calculated with the methylotrophic network, which contains the ethylmalonyl-CoA pathway (EMCP). B: EFMs calculated with the network variant where EMCP was replaced by the glyoxylate cycle (ICL variant).