Table 1 Chemical content and physiological parameters of M. extorquens AM1 cells growing on methanol

Protein

59.13 ± 2.11

This study

M. extorquens AM1

Carbohydrate

16.43 ± 1.09

This study

M. extorquens AM1

   Rhamnose (polymer)

8.92 ± 0.92

This study

M. extorquens AM1

   Glucose (polymer)

5.62 ± 0.52

This study

M. extorquens AM1

   Trehalose

1.22 ± 0.20

This study

M. extorquens AM1

   Glucosamine (polymer)

0.09 ± 0.67

This study

M. extorquens AM1

RNA

8.20 ± 0.68

This study

M. extorquens AM1

Fatty acid

4.95 ± 0.29

This study

M. extorquens AM1

DNA

3.00 -

Neidhart et al.; GC content: Vuilleumier et al. (2009)

E. coli

PHB

2.36 ± 0.05

This study

M. extorquens AM1

Polyamine

0.40 -

Neidhart et al.

E. coli

Carotenoid

0.023 -

Konovalova et al. (2007)

M. extorquens AM1

Intracellular metabolites

2.64 -

Kiefer et al. (2008); Guo et al. (2006); Guo et al. (2007); Vorholt et al. (1998); Crowther et al. (2008)

M. extorquens AM1

Inorganic ions

1.01 -

Neidhart et al.

E. coli

Cofactors

0.22 -

Neidhart et al.

E. coli

SUM

98.36

Physiological parameters

value ± σ

units

Data sources

Organism source

Growth rate

0.168 ± 0.003

h^-1

This study

M. extorquens AM1

Specific methanol uptake rate

15.0 ± 0.25

mmol.g^-1.h^-1

This study

M. extorquens AM1

Specific proton production rate

0.22 ± 0.01

mmol.g^-1.h^-1

This study

M. extorquens AM1

Growth-associated ATP maintenance

59.81 -

mmol.g^-1

Neidhart et al.

E. coli

   Macromolecular building costs

26.65 -

mmol.g^-1

This study

M. extorquens AM1

Non-Growth-associated ATP maintenance

9.5 -

mmol.g^-1.h^-1

Rokem et al. (1978)

Methylobacterium