Skip to main content

Table 1 Chemical content and physiological parameters of M. extorquens AM1 cells growing on methanol

From: Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquensAM1

Macromolecule % Cell Dry Weight ± σ Data source Organism source
Protein 59.13 ± 2.11   This study M. extorquens AM1
Carbohydrate 16.43 ± 1.09   This study M. extorquens AM1
   Rhamnose (polymer) 8.92 ± 0.92   This study M. extorquens AM1
   Glucose (polymer) 5.62 ± 0.52   This study M. extorquens AM1
   Trehalose 1.22 ± 0.20   This study M. extorquens AM1
   Glucosamine (polymer) 0.09 ± 0.67   This study M. extorquens AM1
RNA 8.20 ± 0.68   This study M. extorquens AM1
Fatty acid 4.95 ± 0.29   This study M. extorquens AM1
DNA 3.00 -   Neidhart et al.; GC content: Vuilleumier et al. (2009) E. coli
PHB 2.36 ± 0.05   This study M. extorquens AM1
Polyamine 0.40 -   Neidhart et al. E. coli
Carotenoid 0.023 -   Konovalova et al. (2007) M. extorquens AM1
Intracellular metabolites 2.64 -   Kiefer et al. (2008); Guo et al. (2006); Guo et al. (2007); Vorholt et al. (1998); Crowther et al. (2008) M. extorquens AM1
Inorganic ions 1.01 -   Neidhart et al. E. coli
Cofactors 0.22 -   Neidhart et al. E. coli
SUM 98.36    
Physiological parameters value ± σ units Data sources Organism source
Growth rate 0.168 ± 0.003 h-1 This study M. extorquens AM1
Specific methanol uptake rate 15.0 ± 0.25 mmol.g-1.h-1 This study M. extorquens AM1
Specific proton production rate 0.22 ± 0.01 mmol.g-1.h-1 This study M. extorquens AM1
Growth-associated ATP maintenance 59.81 - mmol.g-1 Neidhart et al. E. coli
   Macromolecular building costs 26.65 - mmol.g-1 This study M. extorquens AM1
Non-Growth-associated ATP maintenance 9.5 - mmol.g-1.h-1 Rokem et al. (1978) Methylobacterium